High-Throughput Crystallization Center

Our Expertise – Your Results

The High-Throughput Crystallization Screening Center performs crystal-growth screening experiments in 1,536-well microassay plates for both soluble and membrane biological macromolecules. You send the sample, we do the rest.

Upcoming Screening Run Dates and Deadlines

March 2018 (updated 01/30/2018)

Membrane Screen

      • Reservation Deadline: Friday, March 2nd
      • Package Acceptance: March 6th – 8th
      • Plate Setup: March 6th – 8th

Standard Screen

      • Reservation Deadline: Friday, March 9th
      • Package Acceptance: March 13th – 22nd
      • Plate Setup: March 13th – 15th
        and March 19th – 22nd

Click ‘Get a Crystal‘ for details.

Staff and Support

From February 2000, the laboratory has been used to set up over 24 million crystallization experiments on >16,000 biological macromolecules. Over 1,000 laboratories worldwide have used and benefited from the service.

  • At the HTCSC 1,536 different chemical conditions are sampled representing many of the commercially available crystallization screens. Each condition is imaged visually over a period of six weeks. Second Order Non-linear Imaging of Chiral Crystals (SONICC) and UV Two Photon Emitted Fluorescence (UV-TPEF) imaging of crystallization screening also take place to identify biological crystals not picked up visually or those obscured by precipitate.
  • Color images, and SONICC and UV-TPEF images are integrated with the analysis software provided as part of the service. Lead crystallization conditions can be identified and the complete set of results used to analyze the samples solubility phase diagram.
  • Current list of crystallization cocktails.

In the Literature!

Botulinum neurotoxins (BoNT) are causative agents of botulism in humans and pose a potent bioterrorism threat. Recently, structures of BoNT serotype A binding metal ions were published in JACS by HTCSC user Karen Allen and her group from Boston University together with the group of Kim Janda at Scripps, revealing that transition metals are potential enzyme inhibitors and provide compelling therapeutic targets. Images from the initial crystallization conditions found at the HTCSC are shown on top. The crystals are difficult to see in the midst of the precipitate in the visible image, but the bright spots in the UV-TPEF and SONICC images reveal protein crystals. The structure in the bottom panel shows BoNT/A binding two copper ions.

This service is for academic and other not-for-profit users only. Our commercial users go through ‘the structure people‘, our friends at HarkerBIO (www.harkerbio.com).