STB 531
Protein Production, Purification, and Crystallization (3 credits)
This course is designed to provide the student with an understanding of the principles and practice of the milligrams-scale production and purification of proteins: techniques to isolate a gene and subsequently clone it into an appropriate expression system; expression protocols in bacterial, yeast, baculovirus, and mammalian systems; theoretical basis and practical applications of protein purification and crystallization. (Three lecture hours per week)

STB 533
Crystallographic Methods of Structural Biology I (3 credits each)
This two-semester sequence of courses provides instruction in the theory and practice of crystallographic diffraction methods for determining the three-dimensional atomic structure of biological macromolecules. The first semester course presents an introduction and overview required for all structural biology graduate students. The second semester course presents a more in-depth treatment intended for students who take up thesis or dissertation research in biological crystallography. (Three lecture hours per week each semester)

STB 530
Mathematics and Physics Topics for Structural Biologists (2 credits)
This course is intended for structural biology graduate students whose undergraduate preparation did not include certain topics in elementary mathematics and physics that are needed to understand the basic processes of biomolecular structure determination by crystallographic diffraction methods. The primer course, or equivalent preparation, is pre- or co-requisite for STB 533, Crystallographic Methods of Structural Biology I. (One lecture hour and one recitation hour per week)

STB 535
Macromolecular Interactions in Solution (3 credits)
Theory and practice of solution NMR and other biophysical methods used in the study of interactions between macromolecules and their ligands. The course will cover basis of NMR Spectroscopy, surface plasmon resonance, fluorescence, analytical ultracentrifugation, calorimetry, single molecule studies and a variety of biophysical techniques. Permission of the instructor is required.

STB ###
Molecular Biology and Biochemistry Topics for Structural Biologists (2 credits)
This course is intended for structural biology graduate students with undergraduate majors in mathematics, physics, chemistry, computer science, or engineering whose background did not include sufficient preparation in molecular biology and biochemistry. This primer course, or equivalent preparation, is prerequisite STB 531, Protein Production, Purification, and Crystallization. (One lecture hour and one recitation hour per week)

In addition, structural biology graduate students are encouraged to enroll in BIO 608 and encouraged to enroll in CHE 512:

BIO 608
Topics in Macromolecular Structure (3 credits)
This advanced course considers the structural, thermodynamic, electrostatic, and dynamic aspects of molecules that direct the folding of proteins and nucleic acids and govern their interactions with each other as well as with small ligands. Students approach these topics with the aid of state-of-the-art molecular graphics and computational chemistry methods. In addition students use bioinformatics approaches to gain insight into the functions of and interrelationships between biological macromolecules.

CHE 512
Advanced Physical Chemistry: NMR and Biomolecular Structure (3 credits)
This course combines a comprehensive theoretical treatment of high resolution NMR spectroscopy with an extensive exposition of the experimental techniques applicable to biological macromolecules. The "Product Operator Formalism" is presented with particular emphasis in order to enable the participants to understand sophisticated multidimensional NMR experiments. This course teaches the methodological foundations to pursue NMR-based structural biology.