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Wolfley using a liquid handling
system to format plates
|Joe Luft examines
The high-throughput screening (HTS) laboratory will prepare crystal-growth screening experiments in 1536-well microassay plates for a nominal fee. (This fee will be waived for academic users requesting the specialized membrane protein crystallization screen.*)
Experiments are started at approximately one-month intervals. Although an effort is made to accommodate everyone as quickly as possible, there is a queue for sample submission, and a delay occasionally occurs. If you are interested in reserving a spot in the crystallization queue, you must first contact the HTS Lab (firstname.lastname@example.org) for information regarding the screening schedule. Once you are informed of the schedule, you will need to complete the HTS Order Form for the standard screen ( located above) or the membrane screen in order to complete the reservation process.
Information will not be publicly disclosed regarding any sample received for crystallization trials without the expressed written consent of the investigator(s). However, investigators will be asked to give certain information about the target protein for inclusion in the crystallization database that is being compiled as part of the ongoing research program in crystal growth at HWI. (Participation is voluntary, but is strongly encouraged and deeply appreciated.) The database is composed largely of results from experiments conducted in the high-throughput laboratory, and it will be used to predict crystallization recipes for proteins that have never been crystallized successfully. The prediction algorithm depends on the identification of proteins that have been crystallized and are similar to the problem at hand. Measures of similarity based on biochemical and biophysical properties are being devised.
If the high-throughput screening facility assists you in identifying initial crystallization conditions, then to help us track the use and success of the screening laboratory, we ask you to please reference one of the following articles:
Standard Screen. Luft, J. R., Collins, R. J., Fehrman, N. A., Lauricella, A. M., Veatch, C. K. & DeTitta, G. T. (2003). A deliberate approach to screening for initial crystallization conditions of biological macromolecules. J. Struct. Biol. 142, 170-179. [PubMed ID: 12718929]
Membrane Screen. Koszelak-Rosenblum, M., Krol, A., Mozumdar, N., Wunsch, K., Ferin, A., Cook, E., Veatch, C. K., Nagel, R., Luft, J. R., DeTitta, G. T & Malkowski, M. G. (2009). Determination and application of empirically derived detergent phase boundaries to effectively crystallize membrane proteins. Protein Science 18, 1828-1839. [Pub Med ID: 19554626]
* Industrial users should contact Mike Malkowski (716-898-8624) for pricing information regarding the membrane screen.
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