W0233

Comparison of Complete Data Sets Acquired with Different Polycapillary Optic Configurations. Frank A. Hofmann¹, Walter M. Gibson¹, Daniel C. Carter², Joseph X. Ho², John R. Ruble², Johannes B. Ullrich³, ¹Center for X-Ray Optics, University at Albany, Albany NY, 12222 USA, ²New Century Pharmaceuticals, Inc., Huntsville AL, 35824 USA, ³X-Ray Optical Systems, Inc., Albany NY, 12203 USA

Polycapillary optics can be utilized to optimize the characteristics of x-ray beams for special applications. Different polycapillary optics configurations have been used successfully in the past for a variety of single crystal diffraction applications. The work described in this paper concerns a systematic study of the application of both collimating and focusing optics to x-ray crystallographic structure determination of selected proteins using a standard rotating anode source.

The results summarize a series of measurements comparing duplicate data sets obtained from an individual crystal. Intensity and data quality are discussed for measurments with pinhole collimators, collimating polycapillary optic, and focusing polycapillary optics. Approaches to optimize data collection strategies for different optic configurations will be discussed. The collected data was analyzed using conventional software. Opportunities and limitations on the use of conventional analysis software for data from focused beams will be discussed.

Future improvements to software and optic configurations have been identified as a result of these measurements. They will be discussed and first experimental evidence of the applicability of these configurations will be presented.

This work was supported by NASA under cooperative agreements NCC-8-125 and NAS8-97247.